Peptide preparation Welcome the wind? It is enough to read this article about domestic polypeptide cdmo

Author Zhiyin capital

Peptides generally refer to a class of compounds formed by dehydration and condensation of 3-50 α – amino acids. According to different sources, they can be divided into three categories: extracted peptides, chemically synthesized peptides (exenatide) and gene recombinant peptides (liraglutide). The advantages of peptide drugs are strong drug-forming, high activity, good specificity, relatively weak toxicity, not easy to accumulate, less cross reaction of drugs, etc.

peptide drugs have the advantages of high biological activity, strong specificity and small side effects. In 2015, the global sales of peptide drugs reached 22 billion US dollars, which is one of the fastest growing drug fields. In recent years, the world’s super varieties of peptides are somaluptide, liraglutide, exenatide, leuprorelin, tripatide With the help of the circle of friends of the screen brushing pharmaceutical people, peptide pharmaceutical enterprises have submitted applications for the science and technology innovation board. Recently, Chengdu shengnuo biological polypeptide pharmaceutical company has submitted an application for science and technology innovation board.

Peptide is the high quality target of cdmo project

It is a very good choice to choose polypeptide as research and development project in cdmo project. From the difficulty of production: small molecule < peptide < antibody; from the difficulty of patent breakthrough: antibody < peptide < small molecule; from the difficulty of generic drug: small molecule < antibody < peptide. Therefore, in view of the difficulty of peptide research and development, production and patent breakthrough, peptide drugs are excellent targets of cdmo project.

In addition, peptide drugs are a huge family. There are hundreds of polypeptide drugs in the research and development stage, and the development fields also extend to various indications. The common fields include anti infection, anti-tumor, physiological regulation, pain, heart failure, osteoporosis, diabetes, vaccine, etc. the synthesis methods and routes of each peptide drug are very different, which gives many peptide cdmo The development of the company provides broad opportunities.

Production of polypeptide — a technical barrier difficult to break through

Taking leuprorelin as an example, the earliest original product was listed in the United States in 1994, and the sustained-release preparation was listed in 2002. The patent period was very early, but it did not affect its sales. In 2000, Takeda’s liangbingruilin entered China, and its trade name was “yinatonong”. There is no patent for this drug in China, but only two generic drugs of domestic enterprises have been approved for listing, which shows the high technical barriers of peptide drug development.

The production of polypeptide mainly includes chemical synthesis and enzymatic method. From synthesis to purification and purification, preparations are all barriers that need to be broken through, and these barriers are not only the key problems of technology and quality, but also the cost control.

Among them, chemical synthesis is considered to be a conventional and widely used technology for polypeptide production, but it lacks specificity and environmental burden However, the disadvantages of this technology are low production efficiency, expensive biocatalyst and lack of validation scheme, which brings challenges to peptide production.

Chemical synthesis is divided into liquid-phase peptide synthesis (LPPS) and solid-phase peptide synthesis (SPPs).

LPPS is carried out in solution, using protected amino acids. After each coupling and deprotection, it needs to be separated from the solution. SPPs can synthesize polypeptide chains on insoluble polyester. After each deprotection, the protected amino acids are added to the resin one by one without intermediate separation steps.

After the required length was synthesized on the resin, the peptide was cut off and purified by chromatography. For the production of specific peptides, it is also possible to use a mixture of liquid-phase synthesis and solid-phase synthesis.

After years of research and development, solid-phase synthesis is widely used, which not only overcomes the time-consuming and cumbersome of liquid-phase synthesis, but also reduces the loss caused by operation. The biggest advantage is that all the purification steps in the synthesis are completed by simple washing and filtration, which greatly reduces the difficulty of purification.

It has the advantages of convenient and fast, simple operation and high yield, making it the preferred method for peptide chemical synthesis. Its characteristics are: 1) high degree of automation, and the product production can be basically completed in one automatic synthesis instrument. 2) The reaction is basically normal temperature and pressure, with high safety. 3) Class a solvents such as acetonitrile should be used in production, so the fire protection category of synthesis section is class A, and the purification section is designed according to different processes, and some of them also need to be designed according to class A.

It can be seen that liquid phase synthesis of short chain polypeptides (< 15 amino acids) for high purity drugs is more economical, so liquid phase peptide synthesis method is usually used to synthesize this peptide on a large scale. Although the production of polypeptides on a large scale is much more economical than that of liquid phase synthesis. However, due to the complexity of the process, liquid phase synthesis is not a feasible choice for long peptide.

The method of using enzyme to catalyze protein is called enzymatic method, which is the preferred choice for the synthesis of long peptide.

The method of enzymatic degradation uses the technology of enzyme to hydrolyze, degrade and hydrolyze the protein to obtain polypeptide with molecular weight ranging from 200 to 1000 Da.

The enzymatic process has the advantages of simple process, low investment, stable quality, high activity, high content and good taste. In the process of enzymatic production, the components of enzymatic hydrolysate are complex. Besides peptides, they contain impurities such as macromolecular protein, enzyme, ash and so on. In order to obtain products with high purity, the impurities can be removed as much as possible and enriched with peptides.

For sale, buy peptides, purchase peptides, and buy peptides online, you can find the Chinese peptide company:

Shengnuo Biotechnology is a high-tech China polypeptide manufacturers,who has over 19 years experience for the biotechnology .Our Products involve peptide raw materials,polypeptide drug.

Our 0 defect has passed the FDA certification, and has become the first-class professional polypeptide drug and product development and large-scale production and export industry in China .

Our service scope：peptide synthesis、peptide hormones、peptide medicine、beauty peptide、peptide medicine product、peptide technology transfer、peptide technology service、peptide large-scale production、export of peptide

Thymopentin for Injection、 Thymalfasin for Injection 、 Bivalirudin 、Bulk Drug、 Liraglutide ……

How many companies are there in peptide api manufacturer in china? The peptide api market is very promising, and the world is encouraging the development of peptide business. There is a peptide api list on the website Biofda.com, which contains various specifications of peptide APIs for customers to choose from. Shengnuo Technology is a peptide api manufacturer located in Chengdu, a city in southwest China. Not only peptide APIs, but also carnosine custom suppliers and cosmetic peptide suppliers

are many peptide apis manufacture in China, but they are all small-scale companies. The China peptide company such as Sinotech is a leading company in China and has a very high position.
As a Chinese peptide company, Sinotech has been working silently, hoping to become a top peptide company in the world. There are many countries producing peptides in the world, such as bulk drug substance in India, gmp custom peptide in uk, and peptide production in usa. So what is polypeptide? What kind of peptide synthesis supplier should you choose? Follow our website: www.biofda.com, here will tell you the answer.